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Bio/dNTP's™

ULTRA PURE Bio/dNTP’s™ are custom manufactured to guarantee batch to batch reproducibility.  This commitment to quality is very important in assays where variables have to be minimised. Each set is supplied with a ‘Quality Assurance’ certificate. The Bio/dNTP’s™ are provided in a ready-to-use liquid form either as a convenient cocktail or with each dNTP as a separate 25µM solution.

Specification

Solution
Empirical Formula
Molecular Weight
dATP (2' Deoxyadenosine-5'-triphosphate, Sodium)
C10H14N5O12P3Na2.3H2O
589.2
dCTP (2'-Deoxycytidine-5'-triphosphate, Sodium)
C9H12N5O13P3Na2.H2O
551.1
dGTP (2'-Deoxyguanosine-5'-triphosphate, Sodium)
C10H12N5O13P3Na3.H2O
591.2
dTTP (2'-Deoxythymidine-5'-triphosphate, Sodium)
C10H14N2O14P3Na3.2H2O
583.09
dUTP (2'-Deoxyuridine-5'-triphosphate, Lithium)
C9H11N2O14P3Li4
491.9

Storage

Short/long term: Store at -20°C
Short term: Diluted working solution may be stored at 4°C for up to 4 weeks

Purity

> 98% by HPLC

Bio/dNTP's™Set

A set of four vials containing 25µmoles of each deoxynucleotide triphosphate, dATP, dCTP, dGTP, dTTP. The dNTP's are in solution and are provided at a concentration of 100mM, pH7

DNA Polymerisation Mix

A DNA polymerisation mix containing equimolar amounts of dATP, dCTP, dGTP and dTTP. The dNTP’s are in solution and are provided at a concentration of 5mM. For primer extension procedure, 4µl in a total of 100µl will give a final concentration of 200µM of each dNTP.

dNTP Set

A set of four vials containing 2.5µmoles of each deoxynucleotide triphosphate, dATP, dCTP, dGTP, dTTP. The dNTP's are in solution and are provided at a concentration of 10mM, pH7

DNA Polymerisation Mix

A DNA polymerisation mix containing equimolar amounts of dATP, dCTP, dGTP and dTTP. The dNTP’s are in solution and are provided at a concentration of 20mM. For primer extension procedure, 1µl in a total of 100µl will give a final concentration of 200µM of each dNTP.

 

300-111 dNTP Set (100mM)
4 x 250µl
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300-112 DNA Polymerisation Mix (5mM)
1 x 1000µl
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300-113 DNA Polymerisation Mix (5mM)
4 x 250µl
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300-114 dNTP Set (10mM)
4 x 250µl
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300-115 DNA Polymerisation Mix (20mM) dATP, dCTP, dGTP, dTTP
1 x 500µl
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300-115U DNA Polymerisation Mix (20mM) dATP, dCTP, dGTP, dUTP
1 x 500µl
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