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SoftGenetics

Softgenetics Mutation Surveyor

SoftGenetics Products - Mutation Surveyor


Mutation Surveyor™ - A Unique Research Tool

Mutation Surveyor is available in 400 and 48 lane capacities. Utilizing our patented anti-correlation technology, the program rapidly locates all differences between the wild type sequence and sample traces with excellent accuracy and sensitivity. The program can be used with either single direction or bi-directional data.

Analysis of Single and Bi-directional Data

Single as well as dual directional sequence traces are easily analyzed for any potential variants using out patented anti-correlation technology which performs an actual physical comparison of the sample/patient traces to the reference traces. Found differences are indicated by the sharp peaks in the mutation electropherogram.
Single as well as dual directional sequence traces are easily analyzed for any potential variants using out patented anti-correlation technology which performs an actual physical comparison of the sample/patient traces to the reference traces. Found differences are indicated by the sharp peaks in the mutation electropherogram.
Single Direction Analysis
Dual Directional Analysis
Detection Sensitivity to 5%
Single as well as dual directional sequence traces are easily analysed for any potential variants using out patented anti-correlation technology which performs an actual physical comparison of the sample/patient traces to the reference traces. Found differences are indicated by the sharp peaks in the mutation electropherogram.
Analysis courtesy of Dr. Yidong Bai, Assistant Professor, University of Texas Health Centre, San Antonio TX.

 

Accuracy of the software in the bi-directional analysis mode is over 99%, with sensitivity to greater than 5% of the primary peak.
Analysis courtesy of Dr. Yidong Bai, Assistant Professor, University of Texas Health Centre, San Antonio TX
Accuracy of the software in the bi-directional analysis mode is over 99%, with sensitivity to greater than 5% of the primary peak.

Researchers no longer need to perform time consuming and inaccurate comparison of the entire sample trace. With Surveyor, any found variants of the sample when compared to the wild type are clearly indicated in our mutation electropherogram as a sharp peak.

Accuracy of the software in the bi-directional analysis mode is over 99%, with sensitivity to greater than 5% of the primary peak. Our collaborators have demonstrated an accuracy of 95% when processing single direction sequence traces.

Surveyor’s detection sensitivity has been enhanced to report similar peaks that are buried in baseline noise of both the forward and reverse traces, alerting researchers to the possible presence of mutations buried in the background noise.

Surveyor easily processes 400 lanes of data in approximately two minutes, and can be operated on a fully automated unattended basis.

The software automatically forms contigs, performs alignments and mutation detection comparing both forward and reverse patient traces to reference or normal traces. Homozygote and Heterozygote mutations are indicated by sharp peaks in our exclusive mutation electropherogram.

Unique algorithms perform a comparison of sample to reference traces in both forward and reverse electropherograms checking for similar peaks that are present in the background. This technique provides almost analytical quality sensitivity.

Homozygote Variants are detected when the color of the reference drops 100% being replaced in the same spatial position by a second color in the sample or patient.
The software detects heterozygote variants by observing a drop in color of in the sample vs. the surrounding peaks and the reference, with a second wave length growing in approximately the same proportion and in the same spatial position.
Homozygote Detection
Homozygote Variants are detected when the colour of the reference drops 100% being replaced in the same spatial position by a second colour in the sample or patient.
Heterozygote Detection
The software detects heterozygote variants by observing a drop in colour of in the sample vs. the surrounding peaks and the reference, with a second wave length growing in approximately the same proportion and in the same spatial position.

Insertions and deletions are found by monitoring the mobility of the sample DNA fragments, again in comparison to the reference, to that of the reference, providing an extremely low false positive rate and ignoring the miscalls or overcalls from basecaller software. In the event that a heterozygote insertion or deletion is detected, the software will de-convolute the multiple patient trace into two clean traces, while continuing with the mutation detection.

Homozygote Variants are detected when the color of the reference drops 100% being replaced in the same spatial position by a second color in the sample or patient.
The software detects heterozygote variants by observing a drop in color of in the sample vs. the surrounding peaks and the reference, with a second wave length growing in approximately the same proportion and in the same spatial position.
Dual Insertion
Heterozygote insertion / deletion
Surveyor and Explorer monitor the migration time of the DNA fragments in comparison to the references. If the program detects a change in the migration time, the Centre line turns from green to red indicating the presence of an insertion or deletion. The software then re-aligns the traces, to calculate the Indel.
The position of the Indel is indicated by the heavy red line.

Assembly of a “whole gene” mutational analysis is easily accomplished with Surveyor. To assemble the mutational analysis of an entire gene, simply enter the Genbank data, pre-analysed mutation projects, and the software will assemble and calculate an allele frequency, providing the locations of the found variants in bp order.

Reporting is extremely user-friendly with several customer customisable reporting formats, or customer can easily cut and paste areas of interest to any graphics capable program such as Microsoft Word. Reports can be easily exported in text, XML or HTML formats. Click here to see examples of the reporting feature.

 Download the DNA Variant Analysis PDF PDF icon (1.18Mb)
 Download the SoftGenetics Application Notes PDF PDF icon (241Kb)

 

MS-001 Mutation Surveyor (400 Lanes) - Academia
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MS-001 Mutation Surveyor (400 Lanes) - Non Academia
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MS-001N Mutation Surveyor (400 Lanes) Network Version & Single User Licence - Academia
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MS-001N Mutation Surveyor (400 Lanes) Network Version & Single User Licence - Non Academia
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MS-002 Additional User Licenses for use with MS-001 - Non Academia
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MS-002 Additional User Licenses for use with MS-001 - Academia
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MS-002N Mutation Surveyor Network Additional User Licences - Academia
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MS-003 Annual updates for subsequent 12 month periods for MS-001 - Academia
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MS-003 Annual updates for subsequent 12 month periods for MS-001 - Non Academia
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MS-004 Mutation Surveyor (48 Lanes) - Academia
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MS-004 Mutation Surveyor (48 Lanes) - Non Academia
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MS-005 Annual updates for additional user licences - Academia
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MS-005 Annual updates for additional user licences - Non Academia
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MS-006 Mutations Surveyor™ Site Licence - Non Academia
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MS-006 Mutations Surveyor™ Site Licence
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MS-009 Annual updates for subsequent 12 month periods for MS-004 - Academia
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